Articles
| Open Access |
DOI:
https://doi.org/10.37547/ijmscr/Volume03Issue03-05
STUDY OF ACUTE TOXICITY AND ANTITUMOR ACTIVITY OF NEW COLCHAMETIN PREPARATION СOLCHAMETIN
Yuldashev Javlonabduraimovich , Samarkand State Medical University, Uzbekistan Ibragimov Shavkat Narzikulovich , Republican Specialized Scientific And Practical Medical Center Of Oncology And Radiology Of The Ministry Of Health Of The Republic Of Uzbekistan Shakhanova Shakhnoza Shavkatovna , Samarkand State Medical University, Uzbekistan Esankulova Bustonoy Sobirovna , Samarkand State Medical University, UzbekistanAbstract
The aim of the study was to study acute toxicity in different methods of administration in mice and rats of a new antitumor preparation "colchametin" (K-2) and its antitumor activity on 2 strains of tumors
Material and research methods. Acute toxicity of the preparation K-2 with a single intraperitoneal administration was carried out according to the Litchfield and Wilcoxon method in 60 white infertile mice weighing 202g. both sexes and 60 male and female rats weighing 14010g 5 animals in each group, a total of 120 mice and rats were used.
The study of antithumor activity was performed on 12 non-fertile and 16 linear mice with transfused tumors S-180 and ACATON, which was injected with preparations on the 4th day after tumour was administered on day 4 after tumour transfusion 10 times. Evaluation of the results was carried out according to standard criteria: inhibition of tumor growth (SRW), body weight and spleen of animals. Differences at p < 0.05 were considered reliable.
Results. Such data at single vnutribryushinny introduction are obtained to mice: the average lethal dose of LD50 makes 890 (-150+172) mg/kg, At oral introduction of medicine K-2 to mice of LD50 is equal to 3250 (-630+650) mg/kg, LD50 at single vnutribryushinny to rats of medicine K-2 LD50 makes 410 (-56+61) mg/kg, LD50 at single oral introduction to rats makes 3250 (-630+650) mg/kg, are defined also at all ways of introduction of LD10, LD16 and LD84 value
The antitumor activity of the drug K-2 on the tumor strain of Sarcoma 180 was high - about 99/90%. On the ACATON tumor, the K-2 effect during intraperitoneal administration was lower and reached 76/75%, however, its effect on both tumors was accompanied by a decrease in hematopoietic indicators.
Conclusions. The study of the acute toxicity of the substance of the preparation K-2 showed that this preparation belongs to class IV of low-toxic compounds. On 2 tumors, the effect of the new drug was high, but hematopoietic indicators were reduced.
Keywords
New antitumor drug colchametin, acute toxicity
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