Colorectal cancer remains one of the leading causes of morbidity and mortality worldwide, highlighting the urgent need for new and effective anticancer agents. This study evaluated the cytotoxic activity of eight aspirin analogues (A1, A2, B1, B2, C1, C2, D1, D2) against human colorectal cancer H630 WT cell lines. The results showed that the degree of growth inhibition varied depending on the structural differences among the compounds. The analogues A2, B1, B2, and D2 demonstrated the most pronounced cytotoxic activity, significantly reducing cell viability. These findings suggest that especially A2, B2, and D2 are promising candidates for further investigation as potential anticancer agents in the treatment of colorectal cancer.

Colorectal cancer, aspirin analogues, cytotoxic evaluation

Australian Journal of General Practice. (2023). Chemoprevention. [online] Available at: https://www1.racgp.org.au/ajgp/2018/december/chemoprevention-a-new-concept-for-cancer-preventio.

2. Bashir, A.I.J. (2017). A novel mechanism for the anti-cancer activity of aspirin and its analogues. wlv.openrepository.com. [online] Available at: https://wlv.openrepository.com/handle/2436/620956.

3. Bowel Cancer UK. (2019). Aspirin | Reduce your risk | About bowel cancer. [online] Available at: https://www.bowelcanceruk.org.uk/about-bowel-cancer/risk-factors/reducing-your-risk/aspirin/.

4. Chan, A.T., Ogino, S. and Fuchs, C.S. (2009). Aspirin use and survival after diagnosis of colorectal cancer. JAMA, [online] 302(6), pp.649–58. doi:https://doi.org/10.1001/jama.2009.1112.

5. Chheda, P.B. (2014). Assessment of the in vitro efficacy of aspirin and aspirin analogues in combination with standard chemotherapeutics in glioma cell lines. [online] clok.uclan.ac.uk. Available at: https://clok.uclan.ac.uk/11162/.

6. Claudius, A.-K., Kankipati, C.S., Kilari, R.S., Hassan, S., Guest, K., Russell, S.T., Perry, C.J., Stark, L.A. and Nicholl, I.D. (2014). Identification of aspirin analogues that repress NF-κB signalling and demonstrate anti-proliferative activity towards colorectal cancer in vitro and in vivo. Oncology Reports, [online] 32(4), pp.1670–1680. doi:https://doi.org/10.3892/or.2014.3373.

7. Din, F.V.N., Theodoratou, E., Farrington, S.M., Tenesa, A., Barnetson, R.A., Cetnarskyj, R., Stark, L., Porteous, M.E., Campbell, H. and Dunlop, M.G. (2010). Effect of aspirin and NSAIDs on risk and survival from colorectal cancer. Gut, [online] 59(12), pp.1670–1679. doi:https://doi.org/10.1136/gut.2009.203000.

8. Garcia-Albeniz, X. and Chan, A.T. (2011). Aspirin for the prevention of colorectal cancer. Best Practice & Research Clinical Gastroenterology, [online] 25(4-5), pp.461–472. doi:https://doi.org/10.1016/j.bpg.2011.10.015.

9. International Aspirin Foundation (2020). The Chemistry of Aspirin | The International Aspirin Foundation. [online] www.aspirin-foundation.mtcdevserver4.com. Available at: https://www.aspirin-foundation.com/history/chemistry/#:~:text=Aspirin%20is%20prepared%20by%20chemical.

10. Ismail, N.I., Othman, I., Abas, F., H. Lajis, N. and Naidu, R. (2020). The Curcumin Analogue, MS13 (1,5-Bis(4-hydroxy-3- methoxyphenyl)-1,4-pentadiene-3-one), Inhibits Cell Proliferation and Induces Apoptosis in Primary and Metastatic Human Colon Cancer Cells. Molecules, 25(17), p.3798. doi:https://doi.org/10.3390/molecules25173798.

11. Kankipati, C.S., Perry, C.J. and Nicholl, I.D. (2014). Abstract 335: Aspirin, di-aspirin and their toxicity to colorectal cancer. Cancer Research, 74(19_Supplement), pp.335–335. doi:https://doi.org/10.1158/1538-7445.am2014-335.

12. Kim, B., Park, S.J., Hong, S.P., Cheon, J.H., Kim, W.H. and Kim, T.I. (2015). The effect of prediagnostic aspirin use on the prognosis of stage III colorectal cancer. International journal of clinical and experimental medicine, [online] 8(8), pp.13435–45. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612965/

13. Nicholl, I. (2011). Activity of aspirin analogues and vanillin in a human colorectal cancer cell line. Oncology Reports. doi:https://doi.org/10.3892/or.2011.1320.

14. uclahealth.org. (2021). Should you take aspirin to prevent colorectal cancer? [online] Available at: https://www.uclahealth.org/news/should-you-take-aspirin-to-prevent-colorectal-cancer#:~:text=But%20for%20certain%20people%2C%20taking.

Sleda, M.A., Albasrawi, H.K. and Timmons, S.C. (2018). Synthesis of Aspirin Analogs for Anticancer and Antibacterial Testing. The FASEB Journal, 32(S1). doi:https://doi.org/10.1096/fasebj.2018.32.1_supplement.531.8.