Phlomoides labiosa, a species belonging to the Lamiaceae family, is traditionally used in Central Asian ethnomedicine, yet its bioactive constituents and bioorganic properties have not been thoroughly investigated. This study focuses on the isolation and bioorganic characterization of active compounds from P. labiosa using an integrated analytical approach. Sequential extraction with ethanol and water was followed by chromatographic separation to obtain distinct phytochemical fractions. Structural elucidation of isolated compounds was carried out using GC–MS, HPLC, FT-IR, UV–Vis, and ¹H/¹³C-NMR spectroscopy. The major identified constituents included flavonoids, phenolic acids, terpenoids, sterols, and alkaloid derivatives. Biological activity assays demonstrated strong antioxidant potential and significant antimicrobial effects against selected bacterial and fungal strains. The results indicate that P. labiosa is a rich source of structurally diverse and biologically active natural molecules. The study highlights the pharmacological relevance of the isolated compounds and provides a scientific basis for their potential application in pharmaceutical and bioorganic chemistry research.

Phlomoides labiosa, bioorganic characterization, isolation of active compounds

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