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American Journal Of Biomedical Science & Pharmaceutical Innovation

Peer Reviewed | Open Access | E-ISSN: 2771-2753
Published Article

Corrective Effect Of Polyphenol R-4 On Calcium Homeostasis And Synaptosomal Function In Rats With Experimentally Induced Hypothyroidism

Corrective Effect Of Polyphenol R-4 On Calcium Homeostasis And Synaptosomal Function In Rats With Experimentally Induced Hypothyroidism

Authors

  • Kazakov I.B.
    Institute of Biophysics and Biochemistry, National University of Uzbekistan, 100174, Tashkent, Uzbekistan
  • Khoshimov N.N.
    Institute of Biophysics and Biochemistry, National University of Uzbekistan, 100174, Tashkent, Uzbekistan
  • Khoshimov N.N.
    Institute of Biophysics and Biochemistry, National University of Uzbekistan, 100174, Tashkent, Uzbekistan
  • Rakhimov R.N.
    Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, 100125, Tashkent, Uzbekistan
  • Saidmurodov S. A.
    Alfraganus University, Tashkent, Uzbekistan
  • Safarova D.Dj.
    Uzbek State University оf Physical Culture and Sport, Tashkent, Chirchik 111709, Uzbekistan

DOI:

https://doi.org/10.37547/ajbspi/Volume05Issue10-12

Keywords:

Hypothyroidism polyphenol R-4 synaptosomes

Abstract

Background: Hypothyroidism is an endocrine disorder characterized by reduced thyroid hormone production, leading to impaired neuronal excitability and calcium signaling.

Objective: This study investigates the corrective effect of polyphenol R-1 on calcium-dependent mechanisms of neuronal activity under experimental hypothyroidism.

Methods: Hypothyroidism was induced in male outbred rats (220–250 g) by oral administration of mercazolil (2.5 mg/100 g) for 21 days. Behavioral, biochemical, and synaptosomal calcium changes were evaluated. Calcium concentrations were measured using Fluo-4 AM fluorescence on a USB 2000 spectrofluorimeter. Polyphenol R-4 (50 mg/kg, intraperitoneal) was administered for 10 days after hypothyroidism induction.

Results: Hypothyroid rats showed lethargy, reduced exploratory activity, decreased T3/T4 levels, elevated TSH, and diminished intracellular [Ca²⁺]in synaptosomes. Treatment with R-4 significantly normalized these changes, restoring calcium levels, metabolic parameters, and cognitive behavior.

Conclusion: Polyphenol R-4 demonstrates a neuroprotective effect by stabilizing calcium homeostasis through modulation of NMDA receptor and VGCC activity, suggesting its potential as a pharmacological regulator of calcium signaling in hypothyroid-associated neurodegeneration.

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