Microbiological Monitoring and Identification of Contaminants in Feed Products Produced by Local Manufacturers in The Tashkent Region

Authors

Kutlieva Guzal Jumaniyazovna

Candidate of Biological Sciences, Institute of Microbiology, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan
Turaeva Bakhora Ismoilovna

Doctor of Philosophy (PhD) in Biological sciences, Institute of Microbiology, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan
Shonakhunov Tulkin Erkinovich

Doctor of Philosophy (PhD) in Biological sciences, Institute of Microbiology, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan

DOI:

https://doi.org/10.37547/ajast/Volume06Issue04-23

Keywords:

Animal feed microbiological safety coliform bacteria

Abstract

A study was conducted to assess the microbiological quality of feed products produced by local manufacturers in Tashkent. The analysis included samples of compound feed, cottonseed cake, sunflower cake, soybean cake, and corn grits (V1–V6). Total bacterial contamination, mold content, and the presence of coliform bacteria were determined.

The total bacterial count exceeded the permissible limits (6.2 × 10⁵ – 8.9 × 10⁵ CFU/g), and most samples also showed elevated levels of mold contamination, particularly in sample V6 (6 × 10⁵ CFU/g). Coliform bacteria were detected in 83% of the samples, indicating sanitary violations.

Identification using the MALDI-TOF MS method revealed the dominance of Bacillus spp., as well as the presence of opportunistic microorganisms, including Staphylococcus, Klebsiella, Enterobacter, and Proteus.

The obtained results indicate heterogeneity in feed quality and highlight the need to strengthen sanitary control during production and storage.

References

Manuela Zadravec, Tomislav Mikuš, Nicolas Pradervand and Igor Ujčič Vrhovnik. Microbiological Quality of Feed //The Open Agriculture Journal. -2023.-V.17. http://dx.doi.org/10.2174/18743315-v17-e230418-2022-66

I. Čabarkapa, B. Kokić, D. Plavšić, D. Ivanov, J. Lević. Microbiological safety of animal feed // Biotechnology in Animal Husbandry. -2009. -№. 25 (5-6), p 1155-1162.

Osman Erkmen. Analysis of cereals and cereal products// Academic press-2022. pp.399-403. https://www.researchgate.net/publication/357843888

Ksenija Nesic, Radmila Mitrovic and Radmila Markovic. Categorization of animal feed according to microbiological quality - preferable improvement in the food chain // Earth and Environmental Science. -2021. -№.012065. 1-7.рр.

Nesic K, Habschied K and Mastanjevic K. Possibilities for the biological control of mycotoxins in food and feed // Toxins. -2021. -№13.(3). 198. https://doi.org/10.3390/toxins13030198

Nesic K, Samanc H, Vujanac I, Prodanovic R, Nesic V, Velebit B and Savic B 2014 Detection of meat and bone meal in cattle feed and ruminal fluid – comparison and combining of microscopy and polymerase chain reaction Anim// Feed Sci. Technol. --2014.-№.187. -P. 86 -90

Markovic R, Petrujkic B and Sefer D. Faktori opasnosti (hazarda) u proizvodnji hrane za životinje i kontrolne mere: Biološke opasnosti (hazardi) Bezbednost hrane za životinje, Fakultet veterinarske medicine Univerzitet u Beogradu. -2010. pp 46 -122.

Gafner J L. Microbiologischequalitat von Futtermitteln Agrarforschung Sweiz. -2012.-№.3 252 -7.

Zadravec M, Mitak M, Jaki Tkalec V and Majnaric D. Mikrobiološka kategorizacija krmivai krmnih smjesa VDLUFA metodom Veterinarska stanica. -2015. -№. 46 (3). 175 -80.

Bojan Golic., Dragan Knežević., Dragan Kasagić. Microbiological safety of feed in year 2022// AgroReS 13. -2024. -P. 2016-221.

Morrow W. E., & Gebreyes W.A. Salmonella enterica in commercial swine feed and subsequent isolation of phenotypically and genotypically related strains from fecal samples //Applied and Environmental Microbiology. -2010. -№. 76. -P. 7188-7193.

Ge. B. La, Fon. P. C., Carter P. J., Mc Dermott S. D., Abbott J., Glenn A., Ayers S. L., Friedman S. L., Paige J. C., Wagner D. D., Zhao S., McDermott P. F., & Rasmussen M.A. Retrospective analysis of Salmonella, Campylobacter, Escherichia coli, and Enterococcus in animal feed ingredients // Foodborne Pathogens and Disease. -2013. -№.10. -P. 684-691.

Shariat N. W., K. M. Feye A. K. Richards, B. Booher, Z. Flores, P. M. Rubinelli, E. G. Olson, and S. C. Ricke. Incidence of Salmonella serovars isolated from commercial animal feed mills in the United States and serovar identiﬁcation using CRISPR analy-sis // J. Appl. Microbiol. -2021. -№.130. -P. 2141–2146.

Molla B., Sterman A., Mathews J., Artuso-Ponte V., Abley M., Farmer W., Rajala-Schultz, Morrow W. E., & Gebreyes W.A. Salmonella enterica in commercial swine feed and subsequent isolation of phenotypically and genotypically related strains from fecal samples // Applied and Environmental Microbiology. -2010. -№. 76. -П. 7188-7193.

Biovet S.A. Microbiological quality evaluation of raw materials and animal feed //Veterinaria Digital. -2022. -П.1-2.

Odds F.C. Sabouraud’s agar // Journal of Medical and Veterinary Mycology. – 1991. – Vol. 29. – P. 355–359. https://doi.org/10.1080/02681219180000581

Kaur H., Singh S., Sharma P. Use of Sabouraud dextrose agar for fungal isolation // Journal of Microbiological Methods. – 2025.

Hatagale V.D., Patil S.S., Kulkarni A.P. Effect of culture media on growth and sporulation of fungi // Journal of Scientific Research and Reports. – 2025. – Vol. 31.

Korkom Y., Demirci E. Comparison of different culture media for isolation of fungi // Turkish Journal of Agricultural Sciences. – 2025.

Siregar S., Rahman A. Comparison of potato dextrose agar and Sabouraud dextrose agar for fungal growth // Medical Journal. – 2024.

Madigan M.T., Bender K.S., Buckley D.H., Sattley W.M., Stahl D.A. Brock biology of microorganisms. – 15th ed. – Pearson. – 2018. https://doi.org/10.1017/9780134261976

Cappuccino J.G., Sherman N. Microbiology: A laboratory manual. – 11th ed. – Pearson. – 2017. –

Atlas R.M. Handbook of microbiological media. – 4th ed. – CRC Press. – 2010. https://doi.org/10.1201/9781439804066

MacFaddin J.F. Biochemical tests for identification of medical bacteria. – 3rd ed. – Lippincott Williams & Wilkins. – 2000.