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International Journal of Medical Sciences And Clinical Research

Peer Reviewed | Open Access | E-ISSN: 2771-2265
Published Article

Consistency and Within-Subject Variation in the Interpretation of von Willebrand Factor Multimers Using Semi-Automated Hydragel Assay

Consistency and Within-Subject Variation in the Interpretation of von Willebrand Factor Multimers Using Semi-Automated Hydragel Assay

Authors

  • Marika Pikta
    MD, PhD, Department of Laboratory Medicine, North Estonia Medical Centre, Tallinn, Estonia, Department of Health Technologies, Tallinn University of Technology, Tallinn, Estonia
  • Timea Szanto
    MD, PhD Coagulation Disorders Unit, Department of Hematology, Comprehensive Cancer Centre, Helsinki University Hospital, Helsinki, Finland
  • Maarja Karu
    MD Department of Hematology and Oncology, Tallinn Children’s Hospital, Tallinn, Estonia
  • Laura Johanna Mettis
    MD Haematology Department, North Estonia Medical Centre, Tallinn, Estonia University of Helsinki, Helsinki, Finland
  • Kadri Saks
    MD Department of Hematology and Oncology, Tallinn Children’s Hospital, Tallinn, Estonia

DOI:

https://doi.org/10.37547/ijmscr/Volume06Issue04-07

Keywords:

Von Willebrand factor von Willebrand factor multimers within-subject variation bleeding disorders

Abstract

Objective: Accurate interpretation of von Willebrand factor multimer (VWF:MM) assays is critical for the diagnosis and classification of von Willebrand disease (VWD). To date, no published data have addressed within-subject variability using the semi-automated Hydragel VWF:MM assay.

Materials and Methods: This study evaluated within-subject variation in VWF:MM patterns among 26 individuals assessed for bleeding disorders. VWF:MM analysis was repeated over time using Hydragel H5VW and H11VW gels (Sebia, France).

Results: Across all patients, repeated VWF:MM assessments demonstrated consistent interpretations. Two exceptions occurred due to sample collection after infusion with factor concentrates. These cases were excluded from the final analysis. The study highlights the importance of treatment history in multimer interpretation.

Conclusion: This is the first report to demonstrate reliable within-subject consistency using the semi-automated Hydragel VWF:MM assay. The findings support its clinical utility for VWD diagnosis and monitoring, provided that timing of sample collection relative to factor treatment is appropriately considered.

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