This article presents comprehensive data regarding the determination of the quantitative and qualitative composition of phenolic compounds within a 96% ethanolic extract derived from the walnut fruit septum (Juglans regia L.), utilizing the method of High-Performance Liquid Chromatography (HPLC). The experimental findings reveal the presence of several key bioactive substances, specifically gallic acid, rutin, salicylic acid, quercetin, and apigenin, within the investigated extract. Conversely, the presence of kaempferol was not detected under the established chromatographic conditions. Among the identified phenolic constituents, gallic acid and rutin were found to be present in relatively higher concentrations compared to other components. The analytical results obtained throughout this study demonstrate that the walnut fruit septum represents a highly promising source of natural antioxidants, possessing significant potential for application as a raw material within the pharmaceutical and food industries.
Determination of The Content of Phenolic Compounds in The Septum of Walnut Fruit Using the HPLC Method
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References
Asqarov, I. R. (2023). Fitoterapiya [Phytotherapy] (pp. 386–487). Fan va texnologiyalar nashriyoti-matbaa uyi.
Kuznetsov, V. V. (1956). O‘zbekistonning yong‘oq mevali o’simliklari [Nut-bearing plants of Uzbekistan]. Toshkent.
Yadrov, A. A. (2020). Orekhoplodnye i subtropicheskie plodovye kul'tury [Nut and subtropical fruit crops]. Tavriya.
Meng, Q., Li, Y., Xiao, T., Zhang, L., & Xu, D. (2017). Antioxidant and antibacterial activities of polysaccharides isolated and purified from Diaphragma juglandis fructus. International Journal of Biological Macromolecules, 105, 431–437. https://doi.org/10.1016/j.ijbiomac.2017.07.054
Wang, D., Mu, Y., Dong, H., Yan, H., Hao, C., Wang, X., & Zhang, L. (2018). Chemical constituents of the ethyl acetate extract from Diaphragma juglandis fructus and their inhibitory activity on nitric oxide production in vitro. Molecules, 23(1), 72. https://doi.org/10.3390/molecules23010072
Meng, Q., Wang, Y., Chen, F., Xiao, T., & Zhang, L. (2018). Polysaccharides from Diaphragma juglandis fructus: Extraction optimization, antitumor, and immune-enhancement effects. International Journal of Biological Macromolecules, 115, 835–845. https://doi.org/10.1016/j.ijbiomac.2018.04.142
Rusu, M. E., Gheldiu, A. M., Mocan, A., Moldovan, C., Popa, D. S., Tomuta, I., & Vlase, L. (2018). Process optimization for improved phenolic compounds recovery from walnut (Juglans regia L.) septum: Phytochemical profile and biological activities. Molecules, 23(11), 2814. https://doi.org/10.3390/molecules23112814.