This paper presents the development of a technology for the aqueous-alcoholic extraction of biologically active substances from a multi-component choleretic herbal collection consisting of calendula officinalis (Calendula officinalis), flowers of common tansy ( Tanacetum vulgare ) and peppermint leaves ( Mentha piperita). The following quantitative indicators were chosen as criteria for the process efficiency: the sum of flavonoids (calculated as rutin) and the content of extractive substances.

A series of laboratory experiments was conducted with varying parameters: ethanol concentration (20–70%), temperature (20–80 ° C), stirring speed (40–160 rpm), and extraction time (15–90 min). Extraction efficiency was found to be most significantly influenced by extractant concentration and temperature. Optimal values for these factors were determined. The maximum yield of total flavonoids and extractive substances was achieved under these conditions, amounting to approximately 92.3% for total flavonoids and 91.7% for extractive substances of the maximum possible values. The obtained results confirm the scientific validity and practical applicability of the proposed technology.

Choleretic collection, flavonoids, extractive substances

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