Multitarget Antithrombotic Effects of Scutellarin On Platelet–Coagulation Hemostasis | American Journal Of Biomedical Science & Pharmaceutical Innovation

Authors

Asatillaev Jahongir

Uzbek State University of Physical Culture and Sport, 111709 Chirchik, Uzbekistan
Khoshimov Nozim

Institute of Biophysics and Biochemistry at the National University of Uzbekistan, 100174 Tashkent, Uzbekistan National University of Uzbekistan named after M. Ulugbek, 100174 Tashkent, Uzbekistan
Mukhtorov Alisher

National University of Uzbekistan named after M. Ulugbek, 100174 Tashkent, Uzbekistan
Mаmаdаminоv Rаhmаtjоn

Nаmаngаn Stаtе Univеrsitу. Namangan region, Namangan, 160119, Uzbekistan
Chanqayev O'ljaboy

Uzbek State University of Physical Culture and Sport, 111709 Chirchik, Uzbekistan
Yuldasheva Nargiza

S.Yu. Yunusov Institute of Chemistry of Plant Substances, Academy of Science s of the Republic of Uzbekistan, 100170 Tashkent, Uzbekistan

DOI:

https://doi.org/10.37547/ajbspi/Volume06Issue04-07

Keywords:

Scutellarin platelet aggregation coagulation

Abstract

Background: Scutellarin, a flavonoid-rich compound derived from Erigeron breviscapus–related plant material, has been reported to exert cardiovascular and antithrombotic effects, but its integrated influence on platelet–coagulation hemostasis remains incompletely characterized. Methods: we analyzed the in vitro effects of scutellarin on thrombin time (TT), activated recalcification time (ART), activated partial thromboplastin time (APTT), prothrombin time (PT), and ADP-induced platelet aggregation, as well as its effects on intracellular Ca2+ signaling in platelets. Results: Scutellarin prolonged TT, ART, APTT, and PT in a concentration-dependent manner, indicating a broad anticoagulant profile spanning both intrinsic and extrinsic/common coagulation pathways. TT increased from a baseline range of 18–25 s to as high as 140 s at 50 μM, while PT reached approximately 85 s at the upper tested concentrations. In platelet studies, scutellarin strongly inhibited the second phase of ADP-induced aggregation and attenuated ADP-evoked intracellular Ca2+ rise by approximately 16–19% without behaving as a simple extracellular Ca2+ chelator. Platelet counts were not significantly reduced. Conclusions: The available experimental data support scutellarin as a multitarget modulator of platelet–coagulation hemostasis with both antiplatelet and anticoagulant properties. The pattern of response is consistent with interference at several levels, including thrombin/fibrin formation, coagulation-complex assembly on phosphatidylserine-rich membranes, and receptor-linked platelet Ca2+ signaling.

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